Patch-Clamp Electrophysiology of Intracellular Ca2+Channels

Topic Introduction Patch-Clamp Electrophysiology of Intracellular Ca Channels

The modulation of cytoplasmic free Ca concentration ([Ca]i) is a universal intracellular signaling pathway that regulates numerous cellular physiological processes. Ubiquitous intracellular Carelease channels localized to the endoplasmic/sarcoplasmic reticulum—inositol 1,4,5-trisphosphate receptor (InsP3R) and ryanodine receptor (RyR) channels—play a central role in [Ca ]i signaling in all anim...

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

Due to its exquisite sensitivity and the ability to monitor and control individual cells at the level of ion channels, patch-clamping is the gold standard of electrophysiology applied to disease models and pharmaceutical screens alike. The method traditionally involves gently contacting a cell with a glass pipette filled by a physiological solution in order to isolate a patch of the membrane un...

Beyond the Patch Clamp: Nanotechnologies for Intracellular Recording

The patch clamp is a fundamental tool for neuroscientists, offering insights that have shaped our understanding of the brain. Advances in nanotechnology suggest that the next generation of recording methods is now within reach. We discuss the complexity and future promise of applying nanoscience to neural recording.

Use of Patch Clamp Electrophysiology to Identify Off-Target Effects of Clinically Used Drugs

Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology

Whole-cell patch-clamp electrophysiological recording is a powerful technique for studying cellular function. While in vivo patch-clamp recording has recently benefited from automation, it is normally performed "blind," meaning that throughput for sampling some genetically or morphologically defined cell types is unacceptably low. One solution to this problem is to use two-photon microscopy to ...